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Binding of cationic polyelectrolytes to pectin in solution and in multilayered structures♣

M. MARUDOVA1,*

Affiliation

  1. Department of Experimental Physics, University of Plovdiv, 24 Tzar Assen Str. 4000, Plovdiv, Bulgaria

Abstract

The binding of two polycations, poly-L-lysine (PLL) and chitosan, to pectin in dilute solutions and in multilayered structures on a solid substrate was investigated. The attractive electrostatic interaction was emphasized as a main factor, affecting the interaction between the examined polyelectrolytes. pH ranges, at which binding caused formation of soluble and insoluble complexes, were qualitatively determined by turbidimetric titration. Potentiometric titration of pectin solutions with PLL or chitosan was used for measuring the concentration of the bound polycation, and for determining the binding isotherm. Surface Plasmon Resonance was used for building-up and monitoring the kinetics of the multilayer deposition. The binding parameters in solutions and multilayers were evaluated by Hill’s equation and Karlsson’s model respectively. In both systems, the binding of PLL to pectin was cooperative, whereas that of chitosan was anti-cooperative. The observed phenomena could be explained by the larger flexibility of the PLL chain. Because of the cooperative effect, the binding constant of the PLL/pectin interaction is higher for concentrated systems..

Keywords

Binding constant, Polyelectrolyte, Multilayer, Cooperative binding, Anticooperative binding.

Submitted at: Nov. 5, 2008
Accepted at: Oct. 3, 2009

Citation

M. MARUDOVA, Binding of cationic polyelectrolytes to pectin in solution and in multilayered structures♣, Journal of Optoelectronics and Advanced Materials Vol. 11, Iss. 10, pp. 1428-1431 (2009)