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Calcium phosphate ceramics for biomedical applications

D. PREDOI1,* , R.A. VATASESCU-BALCAN2, I. PASUK1, R. TRUSCA3, M. COSTACHE2

Affiliation

  1. National Institute of Materials Physics, P.O. Box. MG 07, 077125, Magurele, Romania
  2. Molecular Biology Center, University of Bucharest, 91-95 Splaiul Independenţei, 76201, Bucharest 5, Romania
  3. METAV Research & Development, P.O BOX 22, 020011, 31C.A.Rosetti st., Bucharest-2, Romania

Abstract

Calcium phosphate compounds have been studied for biomedical applications due to chemical and structural similarity to the mineral phase of bone and tooth. The composition, physico-chemical properties, crystal size and morphology of synthetic apatite are extremely sensitive to preparative conditions and sometimes it resulted into non-stoichiometric calcium deficient hydroxyapatite (HAp) powders. The present paper describes the synthesis of calcium phosphate ceramics powders via a sol-gel method. The powders were sintered at 600 and 8000C. X-ray diffraction (XRD), scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDXS) were used for characterization and evaluation of the phase composition, morphology and particle size of samples. The functional group of the coatings were analyzed using Fourier transform infrared (FTIR). The XRD analysis revealed a well crystallized HA structure at both temperatures. At 800oC a small amount of CaO (about 0.4 %) was detected. The mean crystallite size, determined from the breadth of the diffraction lines, increases from 30 nm at 600oC to about 110 nm at 800oC. FTIR spectra showed the presence of various 3− 4 PO and OH- groups present in the powders. Osteoblast cells were used to determine cell proliferation, viability and citotoxicity after interaction with the prepared bioceramics, by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a tetrazole) assay. We note that the viability of HAp-600 starts to decrease after 12h, while the viability of HAp-800 decreases after 6h..

Keywords

Iron-oxide, Calcium phosphate, Nanoparticles, Osteoblasts cells, Biocompatibility.

Submitted at: June 25, 2008
Accepted at: July 10, 2008

Citation

D. PREDOI, R.A. VATASESCU-BALCAN, I. PASUK, R. TRUSCA, M. COSTACHE, Calcium phosphate ceramics for biomedical applications, Journal of Optoelectronics and Advanced Materials Vol. 10, Iss. 8, pp. 2151-2155 (2008)