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Cellulose acetate membranes with controlled porosity and their use for the separation of aminoacids and proteins

S. I. VOICU1,* , C. M. NINCIULEANU1, O. MUHULET1, M. MICULESCU2

Affiliation

  1. University Politehnica from Bucharest, Faculty of Applied Chemistry and Materials Sciences, Department of Analytical Chemistry and Environmental Engineering 1-7 Gheorghe Polizu, Bucharest, 011061, Ro
  2. University Politehnica from Bucharest, Faculty of Materials Science, 313 Splaiul Independentei, Bucharest, 060042, Romania

Abstract

The properties of a polymer membrane can be managed via synthesis of filter materials with controlled porosity. This paper surveys the effect of three cationic surfactants (dimethyl-dioctodecil ammonium bromide, alkyl-benzyl-dimethyl ammonium chloride, N-dodecyl-pyridinium chloride) on the geometry and pore size of cellulose acetate membranes. Porosity control is carried out by adding surfactant to the polymer solution (10 % wt cellulose acetate in N, N-dimethylformamide), followed by membrane coagulation. Synthesized membranes were characterized by scanning electron microscopy to study the surfactant’s influence on porosity. Also the membranes were characterized by measuring the hydrodynamic flow of water and alcohol. The retention capacity was measured for the separation of the two proteins - bovine serum albumin and hemoglobin and for the separation of four amino acids - alanine, phenylalanine, tryptophan and lysine. For the bovine serum albumin retention, the higher rejection degree was shown by the dimethyl-dioctodecil ammonium bromide membrane (91%) and in the case of hemoglobin retention, the same membrane showed a rejection rate of 84%..

Keywords

Cellulose acetate membrane, Surfactant, Proteins, Aminoacids.

Submitted at: Feb. 24, 2014
Accepted at: July 10, 2014

Citation

S. I. VOICU, C. M. NINCIULEANU, O. MUHULET, M. MICULESCU, Cellulose acetate membranes with controlled porosity and their use for the separation of aminoacids and proteins, Journal of Optoelectronics and Advanced Materials Vol. 16, Iss. 7-8, pp. 903-908 (2014)