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Enzymatic control of the bio-inspired nanomaterials at the spectroscopic level

M. V. PUTZ1,* , A.-M. LACRĂMĂ2

Affiliation

  1. Laboratory of Computational and Structural Physical Chemistry, Chemistry Department, West University of Timişoara, Str. Pestalozzi No. 16, Timişoara, RO-300115, Romania
  2. Laboratory of Biochemistry, Chemistry Department, West University of Timişoara, Str. Pestalozzi No. 16, Timişoara, RO-300115, Romania

Abstract

Aiming to improve the control of the biological regulation the fit of the enzymatic kinetics with the absorption spectrometry is unfolded in the frame of Michaelis-Menten mechanisms including inhibitions. In this context, the time-dependent expression of binding substrate concentration is considered through its W-Lambert form. Then, employing the authors’ method of logistical transformations the progress curves of substrate and product reactants are revealed under their analytical expressions from where the specific effective reaction time is extracted as a measure of the ligand interactions. The presented method allows optimum choice of the spectroscopic method when marking biomaterials through the control of inhibited enzymes, depending on the output of the widths of substrate or product molecular absorption which indicate the fast-reaction ranges..

Keywords

Enzymes, Michaelis-Menten kinetics, Inhibition, W-Lambert function, Beer-Lambert spectroscopy.

Submitted at: Nov. 15, 2006
Accepted at: Aug. 18, 2007

Citation

M. V. PUTZ, A.-M. LACRĂMĂ, Enzymatic control of the bio-inspired nanomaterials at the spectroscopic level, Journal of Optoelectronics and Advanced Materials Vol. 9, Iss. 8, pp. 2529-2534 (2007)