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Fusion of unilamellar DMPC vesicles in presence of the sulfoxides: effect of time and concentration

YU. E. GORSHKOVA1,*

Affiliation

  1. Joint Institute for Nuclear Research, Dubna, Russia

Abstract

The influence of the dimethyl sulfoxide (DMSO) and diethyl sulfoxide (DESO) on the structure of the unilamellar vesicles (ULVs) DMPC was investigated by small angle neutron scattering (SANS). The studied values of the DESO and DMSO concentrations covered a range 0.0 ≤ X ≤ 0.4 (X – mole fraction of sulfoxide in sulfoxide/water mixture). It was shown that the ULVs are able to form multilamellar vesicles (MLVs) spontaneously in the presence of the sulfoxides without the application of external forces. Fusion of ULVs DMPC in presence of the sulfoxides depends on two factors: time and concentration. The ULVs exhibit long term stability for concentrations up to X=0.1. The spontaneous formation MLVs was observed at Х = 0.2 in the presence of the both sulfoxides. However, DESO causes the fusion of the ULVs about 1/2 hour after samples preparation, while this process occurs within an hour in the presence of DMSO. Moreover, the investigation in short-term time scale shown that formation of the MLVs take place at XDESO = 0.3 and XDMSO = 0.4. Additionally, the dependence of lipid bilayer thickness on the mole fraction XDESO in the gel and liquid-crystalline phases was determined. It turned out that lipid bilayer thickness decreases linearly with increasing of the DESO mole fraction up to 0.2 in both phases..

Keywords

Unilamellar vesicles, Sulfoxides, SANS.

Submitted at: Aug. 17, 2015
Accepted at: Sept. 9, 2015

Citation

YU. E. GORSHKOVA, Fusion of unilamellar DMPC vesicles in presence of the sulfoxides: effect of time and concentration, Journal of Optoelectronics and Advanced Materials Vol. 17, Iss. 9-10, pp. 1532-1537 (2015)