"

Cookies ussage consent

Our site saves small pieces of text information (cookies) on your device in order to deliver better content and for statistical purposes. You can disable the usage of cookies by changing the settings of your browser. By browsing our site without changing the browser settings you grant us permission to store that information on your device.

I agree, do not show this message again.

In vitro cytotoxicity assessment of second-generation photosensitizers for photodynamic therapy

E. STOYKOVA1,* , K. NEDKOVA1, O. SABOTINOV1, R. ION2, R. ALEXANDROVA3

Affiliation

  1. Central Laboratory for Optical Storage and Processing of Information, Acad. G. Bonchev Str., Bl.101, P.O. Box 95, 1113 Sofia, Bulgaria
  2. Institute for Chemical research - ICECHIM, Chemical Analysis Dept., Splaiul Independentei 202, Bucharest-77208, Romania
  3. Institute of Experimental Pathology and Parasitology, Acad. G. Bonchev Str., Bl. 25, 1113 Sofia, Bulgaria

Abstract

In this study we evaluate the cytotoxic activity of two phthalocyanines (metallo-complexes) with absorption bands in the red part of the spectrum: zinc-tri-sulphonated phthalocyanine (ZnS3Pc) and zinc-tetrasulphonated phthalocyanine (ZnS4Pc) on human (8 MG BA and MCF-7) and animal (LSCC-SF-Mc29 and LSR-SF-SR) tumor cell lines using a neutral red uptake cytotoxicity test. The light source is a laser diode emitting at 672 nm. The experiments are made at fluences in the range (2 – 100 J/cm2) at fluence rate of 120 mW/cm2 chosen after a series of test measurements. The efficacy of both phthalocyanines is evaluated from light dose response curves obtained for drug concentrations from 1 up to 10 μg/ml. Both studied phthalocyanines exhibit effective treatment of the animal cell line LSCC-SF-Mc29 and the human cell line 8MG-BA. In the case of ZnS3Pc, the cytotoxic effect is most distinguished for the tumor line 8MG-BA whereas for ZnS4Pc better results are obtained for LSCC-SF-Mc29. The animal cell line LSR-SF-SR and the human line MCF-7 exhibit much greater resistance to both photosensitizers at low concentration. Phthalocyanines (free-base or metallo-complexes), which are synthetic porphyrin-like dyes are promising candidates among the second-generation PSs due to very large extinction coefficients in the far red (660-700 nm)..

Keywords

Photodynamic therapy, Phthalocyanines, Survival light dose.

Submitted at: Nov. 1, 2006
Accepted at: Feb. 15, 2007

Citation

E. STOYKOVA, K. NEDKOVA, O. SABOTINOV, R. ION, R. ALEXANDROVA, In vitro cytotoxicity assessment of second-generation photosensitizers for photodynamic therapy, Journal of Optoelectronics and Advanced Materials Vol. 9, Iss. 2, pp. 490-493 (2007)