"

Cookies ussage consent

Our site saves small pieces of text information (cookies) on your device in order to deliver better content and for statistical purposes. You can disable the usage of cookies by changing the settings of your browser. By browsing our site without changing the browser settings you grant us permission to store that information on your device.

I agree, do not show this message again.

Interaction between keratin and silver nanoparticles: investigation by spectrofluorimetry

W. T. YANG1,2, B. TENG2, Y. HAN1, R. WU1, W. Y. CHEN2,* , C. GAIDAU3

Affiliation

  1. Department of Public Health, Chengdu Medical College, Chengdu 610500, Sichuan, P. R. China
  2. National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu 610065, Sichuan, P. R. China
  3. Leather and Footwear Research Institute, 93, Ion Minulescu St, sector 3, 031215, Bucharest, Romania

Abstract

In this study, the fluorescence quenching of keratin with silver nanoparticles was investigated by spectrofluorimetry. The interaction mechanism was analyzed by the Stern-Volmer equation. Moreover, the number of binding sites, the binding constant, and the thermodynamic functions of the reaction between keratin and silver nanoparticles at different temperatures were calculated separately. The results show that silver nanoparticles can quench the fluorescence of keratin, and the reaction between silver nanoparticles and keratin is completed in a few seconds. High pH value is beneficial for the reaction between silver nanoparticles and keratin. As to the quenching variety between silver nanoparticles and keratin, it is a static quenching, and the magnitude of the reaction constants is 1010, much larger than that of small molecules with proteins. Also, the main type of the reaction between keratin and silver nanoparticles is a hydrophobic reaction with an endotherm and entropy increase in the process. This study could be helpful to understand the underlying interaction mechanisms between bio-macromolecules and silver nanoparticles..

Keywords

Keratin, Silver nanoparticles, Interaction mechanism, Fluorescence quenching, Reaction constant.

Submitted at: Jan. 17, 2014
Accepted at: Sept. 11, 2014

Citation

W. T. YANG, B. TENG, Y. HAN, R. WU, W. Y. CHEN, C. GAIDAU, Interaction between keratin and silver nanoparticles: investigation by spectrofluorimetry, Journal of Optoelectronics and Advanced Materials Vol. 16, Iss. 9-10, pp. 1159-1164 (2014)