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Microstructuration of protein matrices by laser-induced photochemistry

M. IOSIN1,2,* , O. STEPHAN1, S. ASTILEAN2, A. DUPPERAY3,4, P. L. BALDECKa1

Affiliation

  1. Laboratoire de Spectrométrie Physique, Université Joseph Fourier & CNRS UMR5588; Saint Martin d'Hères, France
  2. Babes-Bolyai University, Faculty of Physics, 3400, Cluj-Napoca, Romania
  3. INSERM, U578, Grenoble, France
  4. Université Grenoble I, Groupe de Recherche sur le Cancer du Poumon, Institut Albert Bonniot, Grenoble, France

Abstract

We report on the laser fabrication of BSA and type I collagen microstructures using Eosin Y or Rose Bengal photo sensitizers. Similar cross-linking results have been obtained by one-photon absorption or two-photon absorption using a green Q-switched microlaser or a near-infrared mode-locked Ti:sapphire laser, respectively. The microstructure resolution depends on the laser power, but is typically limited by the size of the laser beam within the sample. The cross-linking of type I collagen with Rose Bengal is obtained in acidic conditions with a large photosensitizer concentration in the millimole range. The biocompatibility of microstructures was demonstrated by observing the specific adhesion of cells on collagen lines..

Keywords

Two-photon absorption, Cross-linking, protein, Cells adhesion.

Submitted at: Nov. 15, 2006
Accepted at: March 15, 2007

Citation

M. IOSIN, O. STEPHAN, S. ASTILEAN, A. DUPPERAY, P. L. BALDECKa, Microstructuration of protein matrices by laser-induced photochemistry, Journal of Optoelectronics and Advanced Materials Vol. 9, Iss. 3, pp. 716-720 (2007)