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Osteoblast cell activity on calcium phosphate layers grown on glass by a laser-liquid-solid interaction

M. DIMITROVA1,* , L. PRAMATAROVA1, E. PECHEVA1, P. LAQUERRIERE2, P. MONTGOMERY3, A. PETROVA4, G. ALTANKOV5

Affiliation

  1. Institute of Solid State Physics, Bulgarian Academy of Sciences, 72 Tzarigradsko Chaussee blvd., 1784 Sofia, Bulgaria
  2. INSERM ERM 0203 (labo des biomatériaux), IFR53, 1 rue du Marechal Juin, BP 138, 51685 Reims, France
  3. Institut d'Electronique du Solide et des Systemes (InESS), CNRS, 23 rue du Loess, 67037 Strasbourg, France
  4. Space Research Institute, Bulgarian Academy of Sciences, 6 Moskovska str., 1000 Sofia, Bulgaria
  5. Institute of Biophysics, Bulgarian Academy of Sciences, Acad. G. Bonchev str., bl. 21, 1113 Sofia, Bulgaria

Abstract

A calcium phosphate layer was grown on glass by a laser-liquid-solid interaction (LLSI) process in simulated body fluid (SBF). Glass samples with a layer grown by simple soaking in the SBF (i.e. without laser irradiation) were prepared for comparison. Formation of an inhomogeneous calcium phosphate (CaP) layer on both laser-treated and non-treated samples was observed. The results showed that the laser irradiation did not change the layer structure and morphology but yielded the growth of a thicker CaP layer. With increasing load the elasticity and the hardness increased for both laser-treated and non-treated samples. Furthermore, we tested the osteoblast cell activity of the CaP layers grown on the laser-treated and non-treated samples. Toxicity test showed that the viability of the cells on the layer grown by the LLSI process was over 95%. A permanent increase in the cell number was observed for both groups of samples, and it was more stable on the laser-treated surfaces. The latter showed a higher cell number after 7 days of cell culturing. A slower increase, resulting in a lower cell numbers was observed for the samples untreated with laser irradiation..

Keywords

Calcium phosphate, Simulated body fluid, Laser-liquid-solid interaction, Osteoblast cells.

Submitted at: Nov. 28, 2006
Accepted at: Jan. 15, 2007

Citation

M. DIMITROVA, L. PRAMATAROVA, E. PECHEVA, P. LAQUERRIERE, P. MONTGOMERY, A. PETROVA, G. ALTANKOV, Osteoblast cell activity on calcium phosphate layers grown on glass by a laser-liquid-solid interaction, Journal of Optoelectronics and Advanced Materials Vol. 9, Iss. 1, pp. 240-243 (2007)