"

Cookies ussage consent

Our site saves small pieces of text information (cookies) on your device in order to deliver better content and for statistical purposes. You can disable the usage of cookies by changing the settings of your browser. By browsing our site without changing the browser settings you grant us permission to store that information on your device.

I agree, do not show this message again.

Regionally-selective adhesion and growth of human osteoblast-like MG 63 cells on micropatterned fullerene C60 layers

L. GRAUSOVA1, J. VACIK2, P. BILKOVA2, V. VORLICEK3, V. SVORCIK4, D. SOUKUP1, M. BACAKOVA1, V. LISA1, L. BACAKOVA1,*

Affiliation

  1. Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1083, CZ 142 20 Prague 4, Czech Republic
  2. Nuclear Physics Institute, Acad. Sci. CR, CZ 250 68 Rez near Prague, Czech Republic
  3. Institute of Physics, Academy of Sciences of the Czech Republic, Na Slovance 2, CZ 182 21 Prague 8, Czech Republic
  4. Department of Solid State Engineering, Institute of Chemical Technology, Technicka 5, CZ 166 28 Prague 6, Czech Republic

Abstract

Fullerenes C60 were deposited on to microscopic glass coverslips using the Leybold Univex-300 vacuum system through metallic masks with rectangle openings. Below the openings, the C60 formed prominences 128 ± 8 nm (A), 238 ± 3 nm (B), 326 ± 5 nm (C) or 1043 ± 57 nm (D) in height. Human osteoblast-like MG 63 cells in the cultures on samples A, B and C were distributed almost homogeneously, while on samples D, they were localized almost exclusively in the grooves among the prominences. Thus, fullerene C60 films can act as substrates for guided cell adhesion and growth..

Keywords

Carbon nanoparticles, Nanotechnology, Surface microstructure, Surface geometry, Bone tissue engineering, Directed cell growth.

Submitted at: March 1, 2008
Accepted at: July 10, 2008

Citation

L. GRAUSOVA, J. VACIK, P. BILKOVA, V. VORLICEK, V. SVORCIK, D. SOUKUP, M. BACAKOVA, V. LISA, L. BACAKOVA, Regionally-selective adhesion and growth of human osteoblast-like MG 63 cells on micropatterned fullerene C60 layers, Journal of Optoelectronics and Advanced Materials Vol. 10, Iss. 8, pp. 2071-2076 (2008)