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Results of light scattering dynamics analysis of biological fluids

D. CHICEA1,* , L. M. CHICEA2

Affiliation

  1. Physics Department, University Lucian Blaga of Sibiu, Dr. Ion Ratiu Str. 7-9, Sibiu, 550012, Romania
  2. Internal Medicine Department, University Lucian Blaga of Sibiu, Pompeiu Onofreiu Str., Nr.2-4, Sibiu, 550166, România

Abstract

When coherent light passes through a transparent fluid having scattering centers (SC) the result of the far field interference is a “speckled” image. When the target is a biological sample or cells in suspension the image is called biospeckle. In a suspension the SCs have a complex movement of both sedimentation and the Brownian motion. As a result the biospeckle image is not static but presents time fluctuations. Human urine from both healthy patients and patients with different diseases was used as a target for the laser beam. The time variation of the light intensity in the far field speckle image was acquired. The autocorrelation function of the time series was calculated for each sample. The same samples were analyzed with the standard laboratory urine analysis procedure. We found that the autocorrelation time of the healthy patient urine is around 5 seconds. When albumin, pus, epithelial cells, red or white blood cells are present the autocorrelation time decreases to 2-3 seconds. When cells conglomerates, oxalate salt conglomerates or cylinders are present, the autocorrelation time increases. The technique can be used for a faster identification of the presence of individual cells or of conglomerates in urine and as a screening test..

Keywords

Biological fluids, Suspensions, Far field speckle, Autocorrelation time.

Submitted at: Nov. 15, 2006
Accepted at: March 15, 2007

Citation

D. CHICEA, L. M. CHICEA, Results of light scattering dynamics analysis of biological fluids, Journal of Optoelectronics and Advanced Materials Vol. 9, Iss. 3, pp. 694-697 (2007)