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TEM study of the effect of some regular beverages on the human dental enamel: an in vitro study

A. ZAHARIA1, V. CIUPINĂ1,2, G. PRODAN1, A. CARAIANE1

Affiliation

  1. Ovidius University of Constanta, 124 Mamaia Avenue, Constanta, Constanta, Romania 900527
  2. Academy of Romanian Scientists, Splaiul Independentei No. 54, Bucharest 050094, Romania

Abstract

Nineteen enamel dental specimens were prepared using nineteen healthy teeth. The specimens randomly divided in four groups: group B (six specimens) immersed in Burn Energy Drinks, group C (six specimens) immersed in Coca-Cola, group G (six specimens) immersed in Gatorade and control group (1 specimen). For specimens belonging to group B,C and D, the immersion times were: 5,15,30,60,120 and 180 minutes, respectively. Specimens were investigated using Transmission Electron Microscopy (TEM) techniques. To obtain suitable powder, the teeth were grind by means of diamond drill at 5000- 10000 rpm. The powder was milled in an agate mortar and then dispersed in alcohol and put on the formvar covered Cu grid. To obtain morphological images of the specimens, conventional BFTEM investigations were performed in areas with maximum information. The same areas were used to obtain diffraction pattern (SAED), and the high-resolution (HRTEM) images. The crystallite dimension was estimated using Debye Scherrer formulas. It is found that during immersion, crystallite size changes compared with the specimen of control. We assume that this is due to the passage of hydroxyapatite in carbonate-hydroxyapatite in the presence of carbon dioxide released from drinks used. HRTEM images and SAED pattern confirms the occurrence of carbonate-hydroxyapatite crystallites in specimens immersed..

Keywords

Enamel dental specimens, Soda, Hydroxyapatite, Carbonate-hydroxyapatite, TEM, HRTEM, SAED.

Submitted at: Sept. 30, 2014
Accepted at: March 19, 2015

Citation

A. ZAHARIA, V. CIUPINĂ, G. PRODAN, A. CARAIANE, TEM study of the effect of some regular beverages on the human dental enamel: an in vitro study, Journal of Optoelectronics and Advanced Materials Vol. 17, Iss. 3-4, pp. 323-327 (2015)