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Vascular endothelial cells in cultures on nanocomposite silver/hydrocarbon plasma polymer films with antimicrobial activity

L. BACAKOVA1,* , H. KOSHELYEV2, L. NOSKOVA1, A. CHOUKOUROV2, O. BENADA3, A. MACKOVA4, V. LISA1, H. BIEDERMAN2

Affiliation

  1. Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic
  2. Department of Macromolecular Physics, Faculty of Mathematics and Physics, Charles University, V Holesovickach 2, 182 00 Prague 8, Czech Republic
  3. Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic
  4. Nuclear Physics Institute, Academy of Sciences of Czech Republic, 250 68 Rez near Prague, Czech Republic

Abstract

Bioactive hydrocarbon plasma polymer films containing 3, 30 and 39 at.% of Ag in the form of nanoclusters were deposited on microscopic glass slides using an unbalanced magnetron with an Ag target operated in the dc mode in a working gas mixture of n-hexane and argon. The films were seeded with bovine pulmonary artery endothelial cells (line CPAE) or inoculated with bacterium Escherichia coli. The CPAE cells on films with 3 at.% Ag adhered and grew to a similar extent as on the control pure hydrocarbon films or glass slides. By contrast, the CPAE cell adhesion on films with 30 or 39 at.% of Ag was very low, and the cells died before day 7 of cultivation. In bacterial cultures, all silver-containing films attenuated the growth of E. coli as opposed to pure hydrocarbon plasma polymer films..

Keywords

Hard hydrocarbon coating, Nanostructure, Endothelium, Focal adhesion plaques, von Willebrand factor, E. coli.

Submitted at: March 1, 2008
Accepted at: July 10, 2008

Citation

L. BACAKOVA, H. KOSHELYEV, L. NOSKOVA, A. CHOUKOUROV, O. BENADA, A. MACKOVA, V. LISA, H. BIEDERMAN, Vascular endothelial cells in cultures on nanocomposite silver/hydrocarbon plasma polymer films with antimicrobial activity, Journal of Optoelectronics and Advanced Materials Vol. 10, Iss. 8, pp. 2082-2087 (2008)